Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue

US9310302B2 · kind B2 · utility

Assignee

• Ventana Medical Systems, Inc. · US

Inventors

• Karl Garsha · Sahuarita, US
• Gary Pestano · Lafayette, US
• Michael Otter · Tucson, US
• Alexandra Dea Nagy · Oro Valley, US
• Ray B. Nagle · Tucson, US
• Phillip C. Miller · Tucson, US
• Jan Froehlich · Munich, DE
• William Glynn Day · Tucson, US

Key dates

• Filing date: Oct 7, 2010
• Grant date: Apr 12, 2016
• Expiry date: Jul 4, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N21/6456
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Multiple modality contrast can be used to produce images that can be combined and rendered to produce images similar to those produced with wavelength absorbing stains viewed under transmitted white light illumination. Images obtained with other complementary contrast modalities can be presented using engineered color schemes based on classical contrast methods used to reveal the same anatomical structures and histochemistry, thereby providing relevance to medical training and experience. Dark-field contrast images derived from refractive index and fluorescent DAPI counterstain images are combined to produce images similar to those obtained with conventional H&E staining for pathology interpretation. Such multi-modal image data can be streamed for live navigation of histological samples, and can be combined with molecular localizations of genetic DNA probes (FISH), sites of mRNA expression (mRNA-ISH), and immunohistochemical (IHC) probes localized on the same tissue sections, used to evaluate and map tissue sections prepared for imaging mass spectrometry.