Metabolically enhanced cyanobacterium with sequentially inducible production genes for the production of a first chemical compound

US9315820B2 · kind B2 · utility

Assignee

• Algenol Biotech LLC · US

Inventors

• Ulf Dühring · Fredersdorf, DE
• Alexandra Friedrich · Berlin, DE
• Kerstin Baier · Kleinmachnow, DE
• Heike Enke · Berlin, DE
• Dan Kramer · Berlin, DE

Key dates

• Filing date: Jun 30, 2014
• Grant date: Apr 19, 2016
• Expiry date: Jun 30, 2034

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds. Further provisions of the present invention include, inter alia, a method for producing the metabolically enhanced cyanobacterium and a method for producing a first chemical compound by culturing the metabolically enhanced cyanobacterium.