Patent · US Active

Methods for selecting eukaryotic cells expressing a heterologous protein

US9315844B2 · kind B2 · utility

2Cited by

2References

15Claims

0Family size

Assignee

Novartis AG · CH

Inventors

Thomas Jostock · Neuenburg am Rhein, DE
Hans-Peter Knopf · Schallstadt, DE

Key dates

Filing date	Feb 6, 2014
Grant date	Apr 19, 2016
Priority date	—
Expiry date	Feb 17, 2034

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/85
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention pertains to a method for selecting at least one eukaryotic host cell expressing a product of interest. The method entails providing a plurality of host cells which are dependent upon folate uptake and contain polynucleotides encoding for the product of interest and a DHFR enzyme. The cells are cultured in a selective culture medium containing at least an inhibitor of DHFR and folate. The expressed cells are then selected.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.