Enhanced ligation reactions
US9315861B2 · kind B2 · utility
60Cited by
7References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 28, 2012 |
| Grant date | Apr 19, 2016 |
| Priority date | — |
| Expiry date | Dec 19, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2565/537
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.