Method and apparatus for target detection using electrode-bound viruses

US9316608B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Gregory A. Weiss · Irvine, US
• Reginald M. Penner · Newport Beach, US
• Phillip Tam · Walnut, US
• Li-Mei Yang · Willow View Lane, US
• Tyler Brigham · Irvine, US

Key dates

• Filing date: Jul 12, 2013
• Grant date: Apr 19, 2016
• Expiry date: Feb 11, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/3277
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A biosensor that is capable of detecting the presence and/or concentration of an analyte or biomarker includes at least one electrically conductive electrode operatively coupled to an impedance analyzer for measuring the change in the resistive impedance ΔZRe of the electrode in response to an applied alternating current at a plurality of frequencies. In one embodiment, at least one electrode is covered with a self-assembled monolayer that is chemically bonded to a surface. A plurality of virus particles such as phage viruses are immobilized on the self-assembled monolayer and may be exposed to a test or sample solution. The virus particles may be obtained from phage-displayed libraries to detect a wide variety of targets including, for example, DNA, RNA, small molecules, and proteins or polypeptides. In another embodiment, the virus particles are electrostatically bound to a substrate in between a pair of elongated electrodes disposed on a substrate.