Serum-free mammalian cell culture medium, and uses thereof
US9321996B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 10, 2012 |
| Grant date | Apr 26, 2016 |
| Priority date | — |
| Expiry date | Jan 27, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/10051
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.