Compositions and methods for monitoring in real time a nicking amplification reaction comprising a primer oligonucleotide with a 3′—terminal region comprising A2′—modified nucleotide
US9322053B2 · kind B2 · utility
10Cited by
7References
7Claims
0Family size
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Key dates
| Filing date | Jul 1, 2015 |
| Grant date | Apr 26, 2016 |
| Priority date | — |
| Expiry date | Jul 1, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention features compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time comprising one or more primer oligonucleotides comprising a 5′ nicking enzyme recognition site and a 3′-terminal region comprising a 2′-modified nucleotide. These methods are compatible with amplification of target oligonucleotides in a test sample, including biological samples, using a nicking amplification reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.