Patent · US Active

Efficient biomolecule recycling method and system

US9322063B2 · kind B2 · utility

10Cited by
1References
22Claims
0Family size

Assignee

Inventor

Key dates

Filing dateJan 25, 2008
Grant dateApr 26, 2016
Priority date
Expiry dateDec 29, 2034

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6874
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A technique is disclosed for recapturing and recycling biomolecule reagents. The technique may be applied in a range of settings, including biopolymer synthesis, sequencing, and so forth. Biomolecule reagents such as nucleotides and oligonucleotides used to process nucleic acids, which may be marked with fluorescent tags, carry blocking agents, and so forth, are introduced to samples in a sample container. After the desired reaction occurs with some of the biomolecule reagents, such as some of the nucleotides or oligonucleotides, the effluent stream is processed to recapture unreacted biomolecule reagents. These may be separated from other reaction components, and recycled into the same or a different sample container. The recaptured biomolecule reagents may be mixed with additional biomolecule reagents prior to reintroduction to the same or different samples.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.