Genetically engineered Yarrowia lipolytica with enhanced extracellular secretion of α-ketoglutarate
US9328363B2 · kind B2 · utility
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Key dates
| Filing date | Dec 29, 2013 |
| Grant date | May 3, 2016 |
| Priority date | — |
| Expiry date | Feb 11, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides four keto acid transporter encoding sequences selected from 6611 protein coding sequences of Yarrowia lipolytica CLIB122 database. Also provided are recombinant Yarrowia lipolytica strains overexpressing the keto acid transporters, which have increased extracellular secretion of α-ketoglutarate. The present invention can be used to increase extracellular levels of α-ketoglutarate during the fermentation process and lower downstream purification cost for α-ketoglutarate production.
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