Method for mass production of high-purity oligonucleotides
US9328366B2 · kind B2 · utility
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11Claims
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Key dates
| Filing date | Jun 15, 2012 |
| Grant date | May 3, 2016 |
| Priority date | — |
| Expiry date | Jun 15, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B60/04
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Provided is a method of mass-producing high-purity nucleotides including providing a sequencing substrate having a clonal library of oligonucleotides on a solid support, sequencing the clonal library, obtaining measured location data of the solid support on the sequencing substrate, mapping pixel data of a signal generated from the solid support given as a result of the sequencing with the measured location data, extracting the solid support having a desired base sequence from the sequencing substrate using the mapping result, and amplifying an oligonucleotide on the extracted solid support to replicate on a large scale.
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