Method of library preparation avoiding the formation of adaptor dimers
US9328378B2 · kind B2 · utility
48Cited by
18References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 30, 2007 |
| Grant date | May 3, 2016 |
| Priority date | — |
| Expiry date | Oct 10, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6874
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method of preparing a library of template polynucleotides which reduces and/or prevents the formation of adaptor-dimers. The invention also relates to the use of a library of templates prepared using the method of the invention for solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a library of template polynucleotides which have common sequences at their 5′ ends and at their 3′ ends which is substantially free of adaptor-dimers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.