Droplet digital PCR with short minor groove probes
US9328384B2 · kind B2 · utility
2Cited by
4References
15Claims
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Key dates
| Filing date | May 2, 2014 |
| Grant date | May 3, 2016 |
| Priority date | — |
| Expiry date | May 2, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6858
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for the detection of ddPCR assay-generated amplicons by short minor groove binder-fluororophore-oligonucleotide-quencher (MGB-Fl-oligo-Q) probes. The short MGB-Fl-oligo-Q probes not only reduce background, but also show improved mismatch discrimination when compared to the same length non-MGB probes for detecting the ddPCR generated targets at room temperature.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.