Reaction-based fluorescent probe for selective detection of carbon monoxide using metal-mediated carbonylation

US9329191B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Christopher J. Chang · Berkeley, US
• Brian William Michel · Berkeley, US

Key dates

• Filing date: Aug 26, 2013
• Grant date: May 3, 2016
• Expiry date: Aug 26, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/581
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Carbon monoxide (CO) is a member of the gasotransmitter family that includes NO and H2S and is implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. We now report a new type of turn-on fluorescent probe for selective CO detection by exploiting palladium-mediated carbonylation reactivity. The compounds of the invention are capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.