Identification of mycoplasm contamination in biotechnology production using Raman spectroscopy
US9334520B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 27, 2014 |
| Grant date | May 10, 2016 |
| Priority date | — |
| Expiry date | Jun 7, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/30
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Mycoplasma contamination of a known cell line is detected by collecting a Raman spectrum of a targeted volume within a sample, the targeted volume containing a known cell line of interest, obtaining a reference spectrum uniquely associated with the known cell line where the obtained reference spectrum is known to be free of mycoplasma and comparing, using a processing device, the reference spectrum to the collected spectrum. Mycoplasma is further detected by identifying whether there are unnatural molecular compositions within the collected spectrum based upon the comparison of the reference spectrum to the collected spectrum and providing an indication as to whether mycoplasma is detected in the collected Raman spectrum based upon whether unnatural molecular compositions are identified within the collected spectrum.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.