Patent · US Active

CHO/CERT cell lines

US9340592B2 · kind B2 · utility

0Cited by

5References

12Claims

0Family size

Assignee

Boehringer Ingelheim International GmbH · DE

Inventors

Lore Florin · Biberach, DE
Eric Becker · Biberach an der Riß, DE
Hitto Kaufmann · Ulm, DE

Key dates

Filing date	May 4, 2010
Grant date	May 17, 2016
Priority date	—
Expiry date	Aug 30, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K14/47
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a CERT S132 A expression cassette. Those cell lines have improved growth characteristics and high CERT S132A expression levels. The invention especially concerns two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM AC-C2990 (CHO/CERT 2.41). The invention further concerns a method of generating such preferred production host cells and a method of producing proteins using the two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM ACC2990 (CHO/CERT 2.41).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.