Method for detecting the presence of carbapenemase-producing bacteria in a sample
US9340819B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jun 21, 2012 |
| Grant date | May 17, 2016 |
| Priority date | — |
| Expiry date | Jun 21, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for detecting the presence of carbapenemase-producing bacteria in a sample, said method comprising the steps of: a) performing cell lysis on a test sample in order to obtain an enzymatic suspension; b) reacting a fraction of the enzymatic suspension obtained in step a) with a reagent kit, said reagent kit comprising —a carbapenemase substrate selected from the group consisting of carbapenems and cephamycins, —a pH color indicator which will change color when the pH of the reaction mixture is comprised between 6.4 and 8.4, wherein a color change after step b) indicates the presence of carbapenemase-producing bacteria in the test sample. The invention also relates to a reagent kit, to a microtiter plate and to their uses in detecting the presence of carbapenemase producers in a test sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.