Automated methods for isolating regenerative cells from adipose tissue

US9347033B2 · kind B2 · utility

Assignee

• General Electric Company · US

Inventors

• Zaeem Ashraf Khan · Albany, US
• Aaron Joseph Dulgar-Tulloch · Clifton Park, US
• Stefan Rakuff · Clifton Park, US
• Philip Alexander Shoemaker · Scotia, US
• Erik Leeming Kvam · Albany, US
• Xiaohui Chen · Changzhou City, CN
• Jaydeep Roy · Saratoga Springs, US

Key dates

• Filing date: Jul 30, 2015
• Grant date: May 24, 2016
• Expiry date: Jul 30, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2521/00
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

A method of processing an adipose tissue to collect adipose derived regenerative cells is provided, wherein the method comprises providing a vessel comprising a fluid jet mixer, introducing the adipose tissue into the vessel, introducing a buffer solution into the vessel; washing the adipose tissue using the fluid jet mixer; introducing an enzyme solution into the vessel; initiating jet mixing into the vessel comprising the adipose tissue, the enzyme solution, and the buffer solution using the fluid jet mixer to digest the adipose tissue to form a digestion product; phase-separating the digestion product into a digested buoyant fat layer and a non-buoyant aqueous layer; and collecting the non-buoyant aqueous layer comprising the adipose derived regenerative cells. A system of processing an adipose tissue to collect adipose derived regenerative cells is also provided.