Method for producing corneal endothelial cell
US9347042B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 5, 2012 |
| Grant date | May 24, 2016 |
| Priority date | — |
| Expiry date | Oct 5, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/54
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention provides a method of efficiently producing corneal endothelial cells, particularly from corneal stroma or iPS cell-derived neural crest stem cells, a method of producing corneal endothelial cells stably in a large amount by inducing more efficient differentiation of stem cells into corneal endothelial cells, and a medicament containing corneal endothelial cells. The method of inducing differentiation of stem cells into corneal endothelial cells includes a step of culturing the stem cells in a differentiation induction medium containing a GSK3 inhibitor (preferably a GSK3β inhibitor) and retinoic acid, with the differentiation induction medium preferably further containing one or more of TGFb2, insulin, a ROCK inhibitor, and the like.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.