Nucleic acids encoding mutant acyl-CoA:lysophosphatidylcholine acyltransferases

US9347075B2 · kind B2 · utility

Assignee

• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• Michael W. Bostick · Wilmington, US
• Narendra S. Yadav · Wilmington, US
• Hongxiang Zhang · Chadds Ford, US
• Quinn Qun Zhu · West Chester, US

Key dates

• Filing date: Jun 13, 2013
• Grant date: May 24, 2016
• Expiry date: Dec 24, 2033

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Mutant acyl-CoA:lysophosphatidylcholine acyltransferases [“LPCATs”] having the ability to convert acyl-CoA+1-acyl-sn-glycero-3-phosphocholine to CoA+1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) are disclosed herein. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding mutant LPCATs, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant LPCATs in oleaginous yeast, are also disclosed.