Microscope for high spatial resolution imaging a structure of interest in a sample

US9348128B2 · kind B2 · utility

Assignees

• INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE) · FR
• CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · FR
• UNIVERSITE DE PARIS DESCARTES · FR

Inventors

• Marc Guillon · Versailles, FR
• Marcel Lauterbach · Oettingen, DE
• Valentina Emiliant · Paris, FR

Key dates

• Filing date: May 3, 2013
• Grant date: May 24, 2016
• Expiry date: May 3, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6463
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A microscope for high spatial resolution imaging a structure of interest in a sample comprising a substance having a first state with first spectral properties and a second state with second spectral properties, the microscope comprising: an objective-lens assembly, a wave front modulating optical device adapted to spatially vary an intensity of a transfer light beam, a probe detector arranged to detect an optical measurement signal from a portion of the substance in the second state and placed in an area of the transfer light beam with an intensity adapted not to transfer the substance between the first and second states said microscope comprising a phase contrast microscopy system which includes an intensity detector arranged to detect an intensity of an illuminating light beam after said illuminating light beam has passed through the sample, the objective-lens assembly and the wave front modulating optical device.