Conformational probes and methods for sequencing nucleic acids

US9353412B2 · kind B2 · utility

Assignee

• Illumina, Inc. · US

Inventors

• Molly He · San Diego, US
• Cheng-Yao Chen · San Diego, US
• Eric T. Kool · Stanford, US
• Mostafa Ronaghi · Menlo Park, US
• Michael Previte · San Diego, US
• Rigo Pantoja · San Diego, US

Key dates

• Filing date: Jun 16, 2011
• Grant date: May 31, 2016
• Expiry date: Mar 30, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6818
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.