Method for preparing nucleic acid library, its uses and kits

US9359642B2 · kind B2 · utility

Assignee

• BGI SHENZHEN · CN

Inventors

• Xuyang Yin · Nanhu, CN
• Chunlei Zhang · Shanghai, CN
• Hui Jiang · Lo Wu, CN
• Xiuqing Zhang · Hefei, CN
• Shengpei Chen · Nanhu, CN

Key dates

• Filing date: Oct 16, 2012
• Grant date: Jun 7, 2016
• Expiry date: Oct 21, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are a method of constructing a nucleic acid library, a method of determining a nucleic acid sequence of a nucleic acid sample, and a kit thereof. The method of constructing the nucleic acid library includes the following steps: subjecting a nucleic acid sample to a DOP-PCR amplification, to obtain a first PCR amplification product; subjecting the first PCR amplification product to a second PCR amplification using a DOP-Amp primer, to obtain a second PCR amplification product; and subjecting the second PCR amplification product to an adaptor-ligation PCR, to obtain a third PCR amplification product, wherein the third PCR amplification product constitutes the nucleic acid library.