Methods and apparatus for laser scanning structured illumination microscopy and tomography
US9360660B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 24, 2013 |
| Grant date | Jun 7, 2016 |
| Priority date | — |
| Expiry date | Jul 5, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/0691
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Certain examples provide a structured illumination microscopy system. The example system includes a laser source to generate excitation illumination directed toward a target. The example system includes a modulator to modulate the excitation illumination temporally in a controllable spatial pattern to be constructed on the target object to provide sub-diffractional resolution in a lateral direction with respect to the target. The example system includes two synchronized laser scanning mirror units in confocal arrangement, the laser scanning units to be synchronized and controlled by a computing device, a first of the scanning mirror units to receive the modulated excitation illumination and project the modulated excitation illumination on the target object and a second of the scanning mirror units to receive emission fluorescence from the target and project the emission fluorescence. The example system includes a detector to collect emission fluorescence from the target via the second of the scanning mirror units.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.