Integrated systems for the multiplexed amplification and detection of six and greater dye labeled fragments

US9366631B2 · kind B2 · utility

Assignee

• NetBio, Inc. · US

Inventors

• Eugene Tan · Arlington, US
• Heung Chuan Lam · Newton, US
• Valery Leonidovich Bogdanov · Baltimore, US
• Gregory John Kellogg · Somerville, US
• John A. Wright · Billerica, US
• Ulrich Hans Thomann · Stow, US
• Richard F. Selden · Wellesley, US

Key dates

• Filing date: Jul 27, 2011
• Grant date: Jun 14, 2016
• Expiry date: Jul 27, 2031

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/2575
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.