RNA preparations comprising purified modified RNA for reprogramming cells

US9371511B2 · kind B2 · utility

Assignee

• The Trustees of the University of Pennsylvania · US

Inventors

• Katalin Kariko · Rydal, US
• Drew Weissman · Wynnebrook Manor, US
• Gary Dahl · Madison, US
• Anthony Person · Madison, US
• Judith Meis · Fitchburg, US
• Jerome Jendrisak · Middleton, US

Key dates

• Filing date: Jul 16, 2015
• Grant date: Jun 21, 2016
• Expiry date: Jul 16, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/95
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs.