Compositions for detection and analysis of polynucleotides using light harvesting multichromophores
US9371559B2 · kind B2 · utility
55Cited by
38References
8Claims
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Key dates
| Filing date | Jan 23, 2012 |
| Grant date | Jun 21, 2016 |
| Priority date | — |
| Expiry date | Sep 3, 2032 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB82Y15/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor PNA complementary to the target polynucleotide. The sensor PNA comprises a signaling chromophore to absorb energy from the excited multichromophore and emit light in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.