Method for producing intestinal cells
US9376665B2 · kind B2 · utility
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1References
4Claims
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Key dates
| Filing date | Oct 31, 2011 |
| Grant date | Jun 28, 2016 |
| Priority date | — |
| Expiry date | Mar 29, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An object of the present invention is to provide a method of producing intestinal cells by use of pluripotent stem cells as a starting material. According to the present invention, provided is a method of producing intestinal cells, comprising the steps of: (A) inducing differentiation of pluripotent stem cells into definitive endoderm cells; and (B) culturing the definitive endoderm cells in the presence of (2′Z,3′E)-6-bromoindirubin-3′-oxime (BIO) and N-[(3,5-difluorophenyl)acetyl]-L-Ala-2-phenyl-L-Gly-tert-butyl-OH (DAPT) to thereby induce differentiation of the definitive endoderm cells into intestinal cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.