Staphylococcus detection assays
US9376723B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 11, 2010 |
| Grant date | Jun 28, 2016 |
| Priority date | — |
| Expiry date | Sep 26, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are methods, kits, and compositions related to nucleic acid detection that allow the detection and discrimination of various Staphylococcus species and types. In particular, provided herein are assays that allow the detection of MSSA, MSSE, MRSA, MRSE, VRSA, VRSE, and MRSA community strain by detecting the presence of absence of one or more target sequences selected from a list of target genes and sequences that include, but are not limited to: mecA, femA-SA, femA-SE, lukF-PV, lukS-PV, VanA, SCCmec type I, type II, type III, type IV, type V, and type VII. In certain embodiments, asymmetric PCR amplification methods are employed. In other embodiments, probe-target generated signals are detected at least two temperatures to generate a temperature/temperature signal ratio.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.