Method and apparatus for precise selection and extraction of a focused component in isoelectric focusing performed in micro-channels
US9377440B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 3, 2012 |
| Grant date | Jun 28, 2016 |
| Priority date | — |
| Expiry date | Oct 3, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2001/4038
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus and method are disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel, which are in fluid communication with each other at a point of intersection. Means are provided for selectively moving the pattern of separated zones following cIEF to the intersection point, and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte into and then out of the extraction channel for collection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.