Nanocarrier based plant transfection and transduction
US9382548B2 · kind B2 · utility
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9Claims
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Key dates
| Filing date | Dec 20, 2013 |
| Grant date | Jul 5, 2016 |
| Priority date | — |
| Expiry date | Dec 20, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.