Determining the sensitivity of a cell to a drug
US9382568B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Aug 10, 2009 |
| Grant date | Jul 5, 2016 |
| Priority date | — |
| Expiry date | Feb 23, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/025
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides an in vitro method for determining the resistance or sensitivity of a cell line or patient sample to a deoxyribonucleoside kinase-dependent drug, wherein the method comprises the steps of: (i) treating a patient sample or cell line, or a portion thereof, with a deoxyribonucleoside kinase-dependent drug; (ii) lysing the cells of the patient sample or cell line from step (i); (iii) optionally, mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for deoxyribonucleoside kinase; (iv) mixing a portion of the cell lysate from (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase and a deoxyribonucleoside kinase transcription promoter; (v) mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase, a deoxyribonucleoside kinase transcription promoter and a dephosphorylating agent; and (vi) measuring the bioluminescence of each of the mixtures from steps (iii) to (v), wherein the comparative levels of bioluminescence of each of the mixtures provides a m…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.