Method for isolating and purifying RNA from biological materials
US9382576B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 24, 2012 |
| Grant date | Jul 5, 2016 |
| Priority date | — |
| Expiry date | Dec 1, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H1/08
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Includes the following steps: (1) Mix organs and tissues with formamide and monovalent cation salt solution, homogenize them to obtain the dehydrated biological sample; (2) Mix the dehydrated biological sample with monovalent cation salt solution for incubation; (3) Add the monovalent cation salt solution with precipitation effect to the mixture, mix them and centrifuge, then pour the supernatant into another centrifuge tube; (4) Add isopropanol, mix it and centrifuge, and then discard the upper phase liquid, the lower phase liquid and the visible residual impurities between the upper and lower phases to get white RNA precipitate. This method proposed in the invention enables efficient isolation of protein from RNA in the biological samples. It avoids decomposition of RNA by the residual protein that remains in the products; the reagent used in the invention is low-toxicity compound with little hazard to the environment and human body; since the products obtained are not easily decomposed, the products can be transported at long distance and stored at room temperature; the method is easy to operate and no special skill is required for the operator. The equipment used is simple and …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.