Methods for purifying polysaccharides and pharmaceutical compositions and medical devices containing the same
US9388252B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 17, 2015 |
| Grant date | Jul 12, 2016 |
| Priority date | — |
| Expiry date | Feb 17, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC08J2305/12
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods for removing endotoxin from naturally occurring materials, such as polysaccharides (e.g., agarose and/or carrageenan) are described herein. Polysaccharides that are substantially free of endotoxins and uses thereof are also described. The polysaccharide materials can be isolated from microorganisms, multicellular organisms, such as, algae, plants, seaweed, etc. The method involves the use of acidic and basic solutions to hydrolyze the lipid-polysaccharide bond in endotoxins. Cleaving the fatty acid from the polysaccharide reduces the water-solubility of the fatty acid and enables its removal with an organic solvent such as ethanol. The polysaccharide component can also undergo acidic or basic hydrolysis due to the weak glycosidic bond between the sugar rings.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.