Composition for use in mycobacteria diagnosis

US9388400B2 · kind B2 · utility

Assignee

• LYSANDO AG · LI

Inventors

• Stefan Miller · Regensburg, DE
• Robert Fischer · Berlin, DE

Key dates

• Filing date: Jul 1, 2013
• Grant date: Jul 12, 2016
• Expiry date: Jul 1, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/33
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to a composition having the activity of degrading the cell wall of a Mycobacterium species comprising: (a) a first fusion protein including (i) a domain with a first enzymatic activity, the enzymatic activity being at least one or more of the following: N-acetyl-b-D-muramidase (lysozyme, lytic transglycosylase), N-acetyl-b-D-glucosaminidase, N-acetylmuramoyl-L-alanine amidase, L-alanoyl-D-glutamate (LD) endopeptidase, c-D-glutamyl-meso-diaminopimelic acid (DL) peptidase, D-Ala-m-DAP (DD) endopeptidase, or m-DAP-m-DAP (LD) endopeptidase, (ii) at least one peptide stretch fused to the N- or C-terminus of the domain with the first enzymatic activity; and (iii) a protein transduction domain (PTD) being at the N- or C-terminus of the first fusion protein; and (b) a second fusion protein including (i) a domain with a second enzymatic activity, the enzymatic activity being at least one or more of the following: lipolytic activity, cutinase, mycolarabinogalactanesterase, or alpha/beta hydrolase; (ii) at least one peptide stretch fused to the N- or C-terminus of the domain of the second enzymatic activity; and (iii) a protein transduction domain (PTD) being at …