Methods, systems, and compositions for detection of microbial DNA by PCR
US9388458B2 · kind B2 · utility
1Cited by
1References
9Claims
0Family size
Inventors
Key dates
| Filing date | May 18, 2012 |
| Grant date | Jul 12, 2016 |
| Priority date | — |
| Expiry date | Dec 19, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides methods, systems, and compositions for detecting low abundance microbial DNA in a sample by PCR. Methods of the present invention are based on a strategy that tags the 5′-end of the target DNA templates with a non-bacterial tagging sequence so as to set the templates apart from the endogenous contaminants present in the PCR reagents. There is also provided fusion probes for tagging the templates and primer sets to amplify the tagged templates. Systems and kits for facilitating and automating methods of the present invention are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.