Patent · US Active

In vivo method for generating diversity in a protein scaffold

US9394372B2 · kind B2 · utility

5Cited by
1References
18Claims
0Family size

Assignee

Inventor

Key dates

Filing dateOct 16, 2012
Grant dateJul 19, 2016
Priority date—
Expiry dateOct 16, 2032

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K2317/64
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A transgenic non-human animal is provided. In certain embodiments, the animal comprises a genome comprising an immunoglobulin heavy chain locus comprising: a) a transcribed gene encoding a fusion protein comprising, from N-terminus to C-terminus: i. a scaffold comprising a first binding domain; and ii. a heavy chain constant region operably linked to the scaffold; wherein the scaffold is capable of specifically binding to a target in the absence of additional polypeptides; and b) a plurality of pseudogenes that are operably linked to the transcribed gene and that donate, by gene conversion, nucleotide sequence to the part of the transcribed gene that encodes the binding domain.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.