Culture substrate, culture sheet, and cell culture method
US9394514B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 22, 2010 |
| Grant date | Jul 19, 2016 |
| Priority date | — |
| Expiry date | Sep 24, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2535/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a culture sheet which enables technology in which three-dimensional tissues with uniform diameter are formed without applying chemicals to the surface of a culture substrate. A plurality of holes are formed on the culture sheet of the substrate, and nanopillars capable of controlling the adhesiveness or migration of a cell are formed on a culture surface that serves as the bottom surface of each of the holes. The culture surface of each of the holes having a structure in which a partition wall is provided, wherein, by forming the internal nanopillars in the vicinity of the center of each of the holes, the interaction of the disseminated cells can be limited to uniform the size of the three-dimensional structures of the cells to be formed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.