Gene products of Bacillus licheniformis which form odorous substances and improved biotechnological production methods based thereon

US9394528B2 · kind B2 · utility

Assignee

• BASF SE

Inventors

• Cornelius Bessler · Mülheim an der Ruhr, DE
• Jorg Feesche · Erkrath, DE
• Stefan Evers · Mettmann, DE
• Karl-Heinz Maurer · Erkrath, DE
• Armin Ehrenreich · Oebisfelde, DE
• Birgit Veith · Oebisfelde, DE
• Heiko Liesegang · Rinteln, DE
• Anke Henne · Solingen, DE
• Christina Herzberg · Osterode, DE
• Gerhard Gottschalk · Meinerzhagen, DE

Key dates

• Filing date: Dec 27, 2006
• Grant date: Jul 19, 2016
• Expiry date: Jun 8, 2028

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesized via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their…