Phospholipases, nucleic acids encoding them and methods for making and using them

US9394529B2 · kind B2 · utility

Assignee

• DSM IP ASSETS B.V. · NL

Inventors

• Nelson R. Barton · San Diego, US
• Tim Hitchman · Carlsbad, US
• Jonathan Lyon · San Diego, US
• Eileen O'Donoghue · San Diego, US
• Mark A. Wall · San Diego, US

Key dates

• Filing date: Oct 8, 2010
• Grant date: Jul 19, 2016
• Expiry date: Sep 9, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/04
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.