Method of culturing E. coli cells for high density
US9394560B2 · kind B2 · utility
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17Claims
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Key dates
| Filing date | Mar 12, 2013 |
| Grant date | Jul 19, 2016 |
| Priority date | — |
| Expiry date | Mar 12, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N1/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method of culturing E. coli cells for high density, comprising a cell growth step and an expression induction step by which a maximum of cell mass can be obtained with the concomitant maximum expression of a recombinant protein. E. coli transformed to produce a recombinant protein of interest can be grown at a high concentration using the culturing method of the present invention. Therefore, the method increases the productivity of cells as well as the production yield of the recombinant protein, and can be widely applied to the effective production of recombinant proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.