Hetero-assembling, tunable, and injectable hydrogels for cell encapsulation

US9399068B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Sarah C. Heilshorn · Defiance, US
• Widya Mulyasasmita · Mountain View, US
• Lei Cai

Key dates

• Filing date: Apr 20, 2015
• Grant date: Jul 26, 2016
• Expiry date: Apr 20, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/50
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A viscoelastic hydrogel based on a protein hetero-assembled with a polymer is provided. The protein cannot self-assemble with itself and the polymer cannot self-assemble with itself. The protein has a first association sequence (1stA) and a first spacer (1stSp). The polymer has a second association sequence (2ndA) and a second spacer (2ndSp). The first association sequence and the second association sequence are physically cross-linked to interact with each other with a 1:1 known and specific stoichiometry to form a three dimensional scaffold. The protein is represented by {1stA(1stSp)}x1stA, where x is ≧2, and the polymer is represented by {2ndA(2ndSp)}y2ndA, where y≧2.