Media for culturing stem cells
US9404079B2 · kind B2 · utility
7Cited by
28References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 4, 2013 |
| Grant date | Aug 2, 2016 |
| Priority date | — |
| Expiry date | Apr 20, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/52
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Well-defined, xeno-free culture media which comprise a TGF-beta isoform or the chimera formed between IL6 and the soluble IL6 receptor (IL6RIL6), which are capable of mainataining stem cells, and particularly, human embryonic stem cells, in an undifferentiated state are provided. Also provided are cell cultures comprising the culture media and the stem cells and methods of expanding and deriving embryonic stem cells in such well-defined, xeno-free culture media. In addition, the present invention provides methods of differentiating ESCs or EBs formed therefrom for the generation of lineage specific cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.