Method for amplifying NK cells
US9404083B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jun 20, 2012 |
| Grant date | Aug 2, 2016 |
| Priority date | — |
| Expiry date | Oct 11, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/2302
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A technique is needed which can amplify NK cells in vitro and prepare optimum number of NK cells for the adoptive immunotherapy.A method for amplifying NK cells is provided which comprises steps of: preparing cell population which is comprised of NK cells, removing T cells from the cell population which is comprised of NK cells, and, after removal of T cells, cultivating the remaining cells in a medium supplemented with 2500 to 2831 IU/mL of IL-2. The method for amplifying NK cells of the present invention may comprise a step of removing hematopoietic progenitor cells from the cell population. The present invention provides a pharmaceutical composition for adoptive immunotherapy, comprising NK cells which are prepared by the amplifying method of the present invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.