Patent · US Active

Insulin producing cells derived from pluripotent stem cells

US9404087B2 · kind B2 · utility

2Cited by

1References

19Claims

0Family size

Assignee

Kadimastem Ltd. · IL

Inventors

Michel Revel · Rehovot, IL
Judith Chebath · Rehovot, IL
Guy Slutsky · Holon, IL
Alon Levy · Ramat HaSharon, IL
Michal Izrael · Rehovot, IL
Arik Hasson · Kiryat Ono, IL
Kfir Molakandov · Petah Tikva, IL
Rosalia Kaufman · Rehovot, IL

Key dates

Filing date	Dec 15, 2011
Grant date	Aug 2, 2016
Priority date	—
Expiry date	Apr 5, 2032

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2533/74
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method of generating islet cells from pluripotent stem cells is disclosed. The method comprises: (a) culturing the pluripotent stem cells in a differentiation medium so as to differentiate the pluripotent stem cells into endoderm cells; and (b) culturing the endoderm cells in a medium comprising at least one growth factor, a cAMP inducer and retinoic acid (RA), said at least one growth factor being selected from the group consisting of FGF10, bFGF and FGF7 so as to generate further differentiated cells; and (c) culturing the further differentiated cells in a medium comprising a maturation factor selected from the group consisting of nicotinamide, GLP-1 and exendin 4, thereby generating islet cells from pluripotent stem cells. Further methods of generating islet cells are also disclosed, isolated cell populations comprising same and uses thereof.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.