Enhanced heterologous production of lipoxygenases
US9404095B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 28, 2014 |
| Grant date | Aug 2, 2016 |
| Priority date | — |
| Expiry date | Apr 28, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y113/11
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
The invention is directed to the enhanced expression and purification of lipoxygenase enzymes. These enzymes are of wide-spread industrial importance, often produced in heterologous microbial systems. Preferably, the lipoxygenase produced by the methods of the invention is a plant-derived enzyme and expressed at high-levels in a microbial system that includes a protease-deficient host and one or more chaperone expression plasmids. The invention is also directed to amino acid and nucleic acid fragments of the lipoxygenase enzyme including fragments in expression constructs encoding all or a portion of one or more lipoxygenase genes. The invention is also directed to methods of manufacturing bread and other food and also non-food products with lipoxygenase manufactured by the methods of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.