Methods and compositions for universal size-specific PCR
US9404150B2 · kind B2 · utility
53Cited by
108References
34Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 28, 2008 |
| Grant date | Aug 2, 2016 |
| Priority date | — |
| Expiry date | Aug 6, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are products and processes for the amplification, detection and sequencing of short-stranded nucleic acid in the presence of a high background of long-stranded genomic material (e.g., host or maternal nucleic acids). The methods rely on the use of inside and outside primers introduced at varying concentrations, as well as universal amplification reactions that preferentially amplify short, low copy number nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.