Multiplexed analyses of test samples

US9404919B2 · kind B2 · utility

Assignee

• SomaLogic, Inc. · US

Inventors

• Daniel J. Schneider · Arvada, US
• Dan Nieuwlandt · Longmont, US
• Bruce Eaton · Boulder, US
• Martin Stanton · St Andrews, GB
• Shashi Gupta · Worcester, US
• Stephan Kraemer · Boulder, US
• Dominic Zichi · Boulder, US
• Larry Gold · Boulder, US

Key dates

• Filing date: Feb 10, 2014
• Grant date: Aug 2, 2016
• Expiry date: Feb 10, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/74
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non-nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications.