Devices, systems, and methods for microscale isoelectric fractionation
US9409357B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 23, 2013 |
| Grant date | Aug 9, 2016 |
| Priority date | — |
| Expiry date | Mar 8, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/4473
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.