Method of producing recombinant high molecular weight vWF in cell culture
US9409971B2 · kind B2 · utility
7Cited by
16References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 23, 2014 |
| Grant date | Aug 9, 2016 |
| Priority date | — |
| Expiry date | Jul 23, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/24087
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimeric WF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.