Method for constructing libraries of non-standard peptide compounds comprising N-methyl amino acids and other special (non-standard) amino acids and method for searching and identifying active species
US9410148B2 · kind B2 · utility
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Key dates
| Filing date | Sep 8, 2011 |
| Grant date | Aug 9, 2016 |
| Priority date | — |
| Expiry date | Sep 8, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/20
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method for screening a non-standard peptide compound in the peptide library that binds to the target substance, comprising the steps: (i) preparing a non-standard peptide library wherein a special (non-standard) amino acid is randomly incorporated into the peptide sequence by a cell-free (in vitro) translation system comprising a tRNA acylated by a special (non-standard) amino acid; (ii) bringing the obtained peptide library in contact with a target substance; and (iii) selecting a non-standard peptide that binds to the target substance as an active peptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.